PDBe 4tsv

X-ray diffraction
1.8Å resolution

HIGH RESOLUTION CRYSTAL STRUCTURE OF A HUMAN TNF-ALPHA MUTANT

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tumor necrosis factor, soluble form Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 16.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01375 (Residues: 84-233; Coverage: 64%)
Gene names: TNF, TNFA, TNFSF2
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE M18XHF
Spacegroup: R3
Unit cell:
a: 66.72Å b: 66.72Å c: 85.09Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.2 0.262
Expression system: Escherichia coli BL21(DE3)