› 4tlp

X-ray diffraction
1.9Å resolution

Crystal structure of a alanine91 mutant of WCI

Released:
DOI: 10.2210/pdb4tlp/pdb
PDB entry 4tlp coloured by chain, front view.
PDB entry 4tlp coloured by chain, side view.
PDB entry 4tlp coloured by chain, top view.
Source organism: Psophocarpus tetragonolobus
Primary publication:
A conserved tryptophan (W91) at the barrel-lid junction modulates the packing and stability of Kunitz (STI) family of inhibitors.
Majumder S, Khamrui S, Banerjee R, Bhowmik P, Sen U
Biochim Biophys Acta 1854 55-64 (2015)
PMID: 25448016

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145463 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chymotrypsin inhibitor 3 Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 19.34 KDa
Source organism: Psophocarpus tetragonolobus
Expression system: Atlantibacter hermannii
UniProt:
  • Canonical: P10822 (Residues: 26-200; Coverage: 96%)
Sequence domains: Trypsin and protease inhibitor
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 35.29Å b: 69.95Å c: 70.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.205
Expression system: Atlantibacter hermannii

Quick links

Citations

2 citation in other articles

Roles of inter- and intramolecular tryptophan interactions in membrane-active proteins revealed by racemic protein crystallography.
Lander et al. (2023)
1 more