› 4rve

X-ray diffraction
3Å resolution

THE CRYSTAL STRUCTURE OF ECORV ENDONUCLEASE AND OF ITS COMPLEXES WITH COGNATE AND NON-COGNATE DNA SEGMENTS

Released:
DOI: 10.2210/pdb4rve/pdb
PDB entry 4rve coloured by chain, front view.
PDB entry 4rve coloured by chain, side view.
PDB entry 4rve coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The crystal structure of EcoRV endonuclease and of its complexes with cognate and non-cognate DNA fragments.
Winkler FK, Banner DW, Oefner C, Tsernoglou D, Brown RS, Heathman SP, Bryan RK, Martin PD, Petratos K, Wilson KS
EMBO J 12 1781-95 (1993)
PMID: 8491171

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118154 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Type II restriction enzyme EcoRV Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 244 amino acids
Theoretical weight: 28.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P04390 (Residues: 2-245; Coverage: 100%)
Gene name: ecoRVR
Sequence domains: Restriction endonuclease EcoRV
Structure domains: DNA mismatch repair MutH/Restriction endonuclease, type II
DNA (5'-D(*GP*GP*GP*AP*TP*AP*TP*CP*CP*C)-3') Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 10 nucleotides
Theoretical weight: 3.05 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: C2221
Unit cell:
a: 60.2Å b: 78.4Å c: 371.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 not available not available
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

31 review citations

How do site-specific DNA-binding proteins find their targets?
Halford et al. (2004)
30 more

3 mentions without citation

Structure and function of type II restriction endonucleases.
Pingoud et al. (2001)
2 more