› 4rpu

X-ray diffraction
2.26Å resolution

Crystal Structure of Human Presequence Protease in Complex with Inhibitor MitoBloCK-60

Released:
DOI: 10.2210/pdb4rpu/pdb
PDB entry 4rpu coloured by chain, front view.
PDB entry 4rpu coloured by chain, side view.
PDB entry 4rpu coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Mo SM, Liang WG, King JV, Wijaya J, Koehler CM, Tang WJ

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177328 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Presequence protease, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 1014 amino acids
Theoretical weight: 115.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5JRX3 (Residues: 33-1037; Coverage: 97%)
Gene names: KIAA1104, MP1, PITRM1, PREP
Sequence domains:
Structure domains: Metalloenzyme, LuxS/M16 peptidase-like
Presequence protease, mitochondrial Chain: B
Molecule details ›
Chain: B
Length: 1014 amino acids
Theoretical weight: 115.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5JRX3 (Residues: 33-1037; Coverage: 97%)
Gene names: KIAA1104, MP1, PITRM1, PREP
Sequence domains:
Structure domains: Metalloenzyme, LuxS/M16 peptidase-like

Ligands and Environments

5 bound ligands:
Ligand ZN 2 x ZN
Ligand 3UE 4 x 3UE
Ligand GOL 18 x GOL
Ligand ACT 8 x ACT
Ligand CA 2 x CA
3 modified residues:
Ligand MLZ 28 x MLZ
Ligand CAS 2 x CAS
Ligand MLY 46 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 245.624Å b: 85.49Å c: 158.214Å
α: 90° β: 127.53° γ: 90°
R-values:
R R work R free
0.176 0.176 0.208
Expression system: Escherichia coli

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