PDBe 4rfs

X-ray diffraction
3.23Å resolution

Structure of a pantothenate energy coupling factor transporter

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Predicted membrane protein Chain: S
Molecule details ›
Chain: S
Length: 203 amino acids
Theoretical weight: 22.21 KDa
Source organism: Lactobacillus brevis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03SM0 (Residues: 1-203; Coverage: 100%)
Gene name: LVIS_0658
Sequence domains: ECF transporter, substrate-specific component
Energy-coupling factor transporter ATP-binding protein EcfA1 Chain: B
Molecule details ›
Chain: B
Length: 279 amino acids
Theoretical weight: 30.55 KDa
Source organism: Lactobacillus brevis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03PY5 (Residues: 1-279; Coverage: 100%)
Gene names: LVIS_1662, cbiO1, ecfA1
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Energy-coupling factor transporter ATP-binding protein EcfA2 Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 32.18 KDa
Source organism: Lactobacillus brevis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03PY6 (Residues: 1-290; Coverage: 100%)
Gene names: LVIS_1661, cbiO2, ecfA2
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Energy-coupling factor transporter transmembrane protein EcfT Chain: T
Molecule details ›
Chain: T
Length: 280 amino acids
Theoretical weight: 31.94 KDa
Source organism: Lactobacillus brevis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03PY7 (Residues: 1-266; Coverage: 100%)
Gene names: LVIS_1660, ecfT
Sequence domains: Cobalt transport protein

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 77.726Å b: 145.24Å c: 157.141Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.226 0.289
Expression system: Escherichia coli