PDBe 4rdi

X-ray diffraction
1.95Å resolution

Crystal structure of E. coli tRNA N6-threonylcarbamoyladenosine dehydratase, TcdA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA threonylcarbamoyladenosine dehydratase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 288 amino acids
Theoretical weight: 31.24 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q46927 (Residues: 1-268; Coverage: 100%)
Gene names: JW2783, b2812, csdL, tcdA, ygdL
Sequence domains: ThiF family
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P21
Unit cell:
a: 65.71Å b: 97.036Å c: 84.215Å
α: 90° β: 111.83° γ: 90°
R-values:
R R work R free
0.159 0.156 0.209
Expression system: Escherichia coli BL21(DE3)