PDBe 4r9u

X-ray diffraction
2.78Å resolution

Structure of vitamin B12 transporter BtuCD in a nucleotide-bound outward facing state

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vitamin B12 import ATP-binding protein BtuD Chains: C, D
Molecule details ›
Chains: C, D
Length: 249 amino acids
Theoretical weight: 27.09 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P06611 (Residues: 1-249; Coverage: 100%)
Gene names: JW1699, b1709, btuD
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Vitamin B12 import system permease protein BtuC Chains: A, B
Molecule details ›
Chains: A, B
Length: 333 amino acids
Theoretical weight: 35.48 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P06609 (Residues: 1-326; Coverage: 100%)
Gene names: JW1701, b1711, btuC
Sequence domains: FecCD transport family
Structure domains: ABC transporter involved in vitamin B12 uptake, BtuC

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 372.85Å b: 113.21Å c: 69.43Å
α: 90° β: 94.92° γ: 90°
R-values:
R R work R free
0.218 0.217 0.246
Expression system: Escherichia coli