› 4r37

X-ray diffraction
1.9Å resolution

Crystal structure analysis of LpxA, a UDP-N-acetylglucosamine acyltransferase from Bacteroides fragilis 9343 with UDP-GlcNAc

Released:
DOI: 10.2210/pdb4r37/pdb
PDB entry 4r37 coloured by chain, front view.
PDB entry 4r37 coloured by chain, side view.
PDB entry 4r37 coloured by chain, top view.
Source organism: Bacteroides fragilis NCTC 9343
Primary publication:
Structures of Bacteroides fragilis uridine 5'-diphosphate-N-acetylglucosamine (UDP-GlcNAc) acyltransferase (BfLpxA).
Ngo A, Fong KT, Cox DL, Chen X, Fisher AJ
Acta Crystallogr D Biol Crystallogr 71 1068-76 (2015)
PMID: 25945572

Function and Biology Details

Reaction catalysed: 
A (3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = an [acyl-carrier-protein] + a UDP-3-O-(3-hydroxyacylyl)-N-acetyl-alpha-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177500 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP N-acetylglucosamine O-acyltransferase C-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 275 amino acids
Theoretical weight: 29.91 KDa
Source organism: Bacteroides fragilis NCTC 9343
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5LH16 (Residues: 1-255; Coverage: 100%)
Gene names: BF9343_0789, lpxA
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand UD1 1 x UD1
Ligand PE5 1 x PE5
Ligand PG0 1 x PG0
Ligand ACT 2 x ACT
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P4132
Unit cell:
a: 149.403Å b: 149.403Å c: 149.403Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.147 0.185
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Discovery of dual-activity small-molecule ligands of Pseudomonas aeruginosa LpxA and LpxD using SPR and X-ray crystallography.
Kroeck et al. (2019)

3 mentions without citation

Heritability of metabolic syndrome traits in a large population-based sample.
van Dongen et al. (2013)
2 more