› 4qju

X-ray diffraction
2.16Å resolution

Crystal structure of DNA-bound nucleoid associated protein, SAV1473

Released:
DOI: 10.2210/pdb4qju/pdb
PDB entry 4qju coloured by chain, front view.
PDB entry 4qju coloured by chain, side view.
PDB entry 4qju coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Primary publication:
β-Arm flexibility of HU from Staphylococcus aureus dictates the DNA-binding and recognition mechanism.
Kim DH, Im H, Jee JG, Jang SB, Yoon HJ, Kwon AR, Kang SM, Lee BJ
Acta Crystallogr D Biol Crystallogr 70 3273-89 (2014)
PMID: 25478845

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118018 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA-binding protein HU Chains: A, B
Molecule details ›
Chains: A, B
Length: 98 amino acids
Theoretical weight: 10.71 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99U17 (Residues: 1-90; Coverage: 100%)
Gene names: SAV1473, hup
Sequence domains: Bacterial DNA-binding protein
Structure domains: IHF-like DNA-binding proteins
DNA (5'-D(*TP*GP*CP*TP*TP*AP*TP*CP*AP*AP*TP*TP*TP*GP*TP*TP*GP*CP*AP*CP*C)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 21 nucleotides
Theoretical weight: 6.38 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P212121
Unit cell:
a: 39.468Å b: 85.835Å c: 92.708Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.188 0.249
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Recent Developments and Applications of the MMPBSA Method.
Wang et al. (2017)
2 more

9 mentions without citation

Studying protein-DNA interactions using atomic force microscopy.
Beckwitt et al. (2018)
8 more