› 4qjn

X-ray diffraction
2.61Å resolution

Crystal structure of apo nucleoid associated protein, SAV1473

Released:
DOI: 10.2210/pdb4qjn/pdb
PDB entry 4qjn coloured by chain, front view.
PDB entry 4qjn coloured by chain, side view.
PDB entry 4qjn coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Primary publication:
β-Arm flexibility of HU from Staphylococcus aureus dictates the DNA-binding and recognition mechanism.
Kim DH, Im H, Jee JG, Jang SB, Yoon HJ, Kwon AR, Kang SM, Lee BJ
Acta Crystallogr D Biol Crystallogr 70 3273-89 (2014)
PMID: 25478845

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189397 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding protein HU Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 98 amino acids
Theoretical weight: 10.71 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99U17 (Residues: 1-90; Coverage: 100%)
Gene names: SAV1473, hup
Sequence domains: Bacterial DNA-binding protein
Structure domains: IHF-like DNA-binding proteins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 36.626Å b: 82.938Å c: 60.717Å
α: 90° β: 94.89° γ: 90°
R-values:
R R work R free
0.212 0.207 0.263
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Recent Developments and Applications of the MMPBSA Method.
Wang et al. (2017)
4 more

3 mentions without citation

HU of Streptococcus pneumoniae Is Essential for the Preservation of DNA Supercoiling.
Ferrándiz et al. (2018)
2 more