› 4qgk

X-ray diffraction
2.1Å resolution

Structure of the Human Sjogren Larsson Syndrome enzyme fatty aldehyde dehydrogenase (FALDH)

Released:
DOI: 10.2210/pdb4qgk/pdb
PDB entry 4qgk coloured by chain, front view.
PDB entry 4qgk coloured by chain, side view.
PDB entry 4qgk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A gatekeeper helix determines the substrate specificity of Sjögren-Larsson Syndrome enzyme fatty aldehyde dehydrogenase.
Keller MA, Zander U, Fuchs JE, Kreutz C, Watschinger K, Mueller T, Golderer G, Liedl KR, Ralser M, Kräutler B, Werner ER, Marquez JA
Nat Commun 5 4439 (2014)
PMID: 25047030

Function and Biology Details

Reactions catalysed: 
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
(2E,6E)-farnesal + NAD(+) + H(2)O = (2E,6E)-farnesoate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156380 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde dehydrogenase family 3 member A2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 461 amino acids
Theoretical weight: 52.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P51648 (Residues: 1-460; Coverage: 95%)
Gene names: ALDH10, ALDH3A2, FALDH
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1, ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 80.137Å b: 98.582Å c: 145.703Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.223
Expression system: Escherichia coli

Quick links

Citations

7 review citations

The widespread role of non-enzymatic reactions in cellular metabolism.
Keller et al. (2015)
6 more

9 mentions without citation

A gatekeeper helix determines the substrate specificity of Sjögren-Larsson Syndrome enzyme fatty aldehyde dehydrogenase.
Keller et al. (2014)
8 more