› 4qbg

X-ray diffraction
1.37Å resolution

Crystal structure of a stable adenylate kinase variant AKlse4

Released:
DOI: 10.2210/pdb4qbg/pdb
PDB entry 4qbg coloured by chain, front view.
PDB entry 4qbg coloured by chain, side view.
PDB entry 4qbg coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Effectiveness and limitations of local structural entropy optimization in the thermal stabilization of mesophilic and thermophilic adenylate kinases.
Moon S, Bannen RM, Rutkoski TJ, Phillips GN, Bae E
Proteins 82 2631-42 (2014)
PMID: 24931334

Function and Biology Details

Reaction catalysed: 
ATP + AMP = 2 ADP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147678 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylate kinase Chain: B
Molecule details ›
Chain: B
Length: 217 amino acids
Theoretical weight: 24.44 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: P16304 (Residues: 1-217; Coverage: 100%)
Gene names: BSU01370, adk
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand AP5 1 x AP5
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: C2
Unit cell:
a: 68.817Å b: 71.02Å c: 45.555Å
α: 90° β: 95.13° γ: 90°
R-values:
R R work R free
0.181 0.179 0.22
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Structure determination of high-energy states in a dynamic protein ensemble.
Stiller et al. (2022)
3 more