› 4q47

X-ray diffraction
2.9Å resolution

Structure of the DrRecQ Catalytic Core in complex with ADP

Released:
DOI: 10.2210/pdb4q47/pdb
PDB entry 4q47 coloured by chain, front view.
PDB entry 4q47 coloured by chain, side view.
PDB entry 4q47 coloured by chain, top view.
Source organism: Deinococcus radiodurans R1
Primary publication:
Crystal structure of Deinococcus radiodurans RecQ helicase catalytic core domain: the interdomain flexibility.
Chen SC, Huang CH, Yang CS, Way TD, Chang MC, Chen Y
Biomed Res Int 2014 342725 (2014)
PMID: 25243132

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193282 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent DNA helicase RecQ Chains: A, B
Molecule details ›
Chains: A, B
Length: 525 amino acids
Theoretical weight: 58.41 KDa
Source organism: Deinococcus radiodurans R1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RUU2 (Residues: 1-517; Coverage: 63%)
Gene name: DR_1289
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 2 x ADP
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P212121
Unit cell:
a: 84.756Å b: 95.61Å c: 183.832Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.222 0.295
Expression system: Escherichia coli

Quick links

Citations

1 review citation

RecQ helicases in DNA repair and cancer targets.
Newman et al. (2020)

4 mentions without citation

A Decade of Biochemical and Structural Studies of the DNA Repair Machinery of Deinococcus radiodurans: Major Findings, Functional and Mechanistic Insight and Challenges.
Timmins et al. (2016)
3 more