PDBe 4q16

X-ray diffraction
2.6Å resolution

Structure of NAD+ Synthetase from Deinococcus radiodurans

Released:
Source organism: Deinococcus radiodurans R1
Entry authors: Lee JY, Park YW

Function and Biology Details

Reaction catalysed:
ATP + deamido-NAD(+) + NH(3) = AMP + diphosphate + NAD(+)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NH(3)-dependent NAD(+) synthetase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 287 amino acids
Theoretical weight: 31.11 KDa
Source organism: Deinococcus radiodurans R1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RYV5 (Residues: 1-287; Coverage: 100%)
Gene names: DR_A0201, nadE
Sequence domains: NAD synthase
Structure domains: HUPs

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P212121
Unit cell:
a: 113.23Å b: 114.15Å c: 121.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.239 0.293
Expression system: Escherichia coli