› 4pvh

X-ray diffraction
1.4Å resolution

Phl p 4 N158H variant, a glucose dehydrogenase

Released:
DOI: 10.2210/pdb4pvh/pdb
PDB entry 4pvh coloured by chain, front view.
PDB entry 4pvh coloured by chain, side view.
PDB entry 4pvh coloured by chain, top view.
Source organism: Phleum pratense
Primary publication:
Rationally engineered flavin-dependent oxidase reveals steric control of dioxygen reduction.
Zafred D, Steiner B, Teufelberger AR, Hromic A, Karplus PA, Schofield CJ, Wallner S, Macheroux P
FEBS J 282 3060-74 (2015)
PMID: 25619330

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109786 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-binding PCMH-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 500 amino acids
Theoretical weight: 55.75 KDa
Source organism: Phleum pratense
Expression system: Komagataella pastoris
UniProt:
  • Canonical: B2ZWE9 (Residues: 1-500; Coverage: 100%)
Gene name: phlp4
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand FAD 1 x FAD
Ligand NA 2 x NA
Ligand MLI 2 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P6122
Unit cell:
a: 118.04Å b: 118.04Å c: 201.11Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.146 0.144 0.167
Expression system: Komagataella pastoris

Quick links

Citations

2 review citations

Sweating the assets of flavin cofactors: new insight of chemical versatility from knowledge of structure and mechanism.
Leys et al. (2016)
1 more

1 mention without citation

Rationally engineered flavin-dependent oxidase reveals steric control of dioxygen reduction.
Zafred et al. (2015)