› 4prx

X-ray diffraction
1.8Å resolution

E. coli GyrB 43-kDa N-terminal fragment in complex with ADP+Pi

Released:
DOI: 10.2210/pdb4prx/pdb
PDB entry 4prx coloured by chain, front view.
PDB entry 4prx coloured by chain, side view.
PDB entry 4prx coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of the N-terminal Gyrase B fragment in complex with ADP⋅Pi reveals rigid-body motion induced by ATP hydrolysis.
Stanger FV, Dehio C, Schirmer T
PLoS One 9 e107289 (2014)
PMID: 25202966

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142497 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA gyrase subunit B Chain: A
Molecule details ›
Chain: A
Length: 398 amino acids
Theoretical weight: 44.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AES6 (Residues: 2-392; Coverage: 49%)
Gene names: JW5625, acrB, b3699, cou, gyrB, himB, hisU, nalC, parA, pcbA
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ADP 1 x ADP
Ligand PO4 1 x PO4
Ligand MG 1 x MG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 77.64Å b: 131.65Å c: 92.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.163 0.203
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The role of ATP-dependent machines in regulating genome topology.
Hauk et al. (2016)
2 more

4 mentions without citation

Structure of the N-terminal Gyrase B fragment in complex with ADP⋅Pi reveals rigid-body motion induced by ATP hydrolysis.
Stanger et al. (2014)
3 more