› 4pnr

X-ray diffraction
1.71Å resolution

Crystal Structure of human Tankyrase 2 in complex with TIQ-A.

Released:
DOI: 10.2210/pdb4pnr/pdb
PDB entry 4pnr coloured by chain, front view.
PDB entry 4pnr coloured by chain, side view.
PDB entry 4pnr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights into the binding of PARP inhibitors to the catalytic domain of human tankyrase-2.
Qiu W, Lam R, Voytyuk O, Romanov V, Gordon R, Gebremeskel S, Vodsedalek J, Thompson C, Beletskaya I, Battaile KP, Pai EF, Rottapel R, Chirgadze NY
Acta Crystallogr D Biol Crystallogr 70 2740-53 (2014)
PMID: 25286857

Function and Biology Details

Reaction catalysed: 
NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190549 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly [ADP-ribose] polymerase tankyrase-2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 227 amino acids
Theoretical weight: 25.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H2K2 (Residues: 959-1164; Coverage: 18%)
Gene names: PARP5B, TANK2, TNKL, TNKS2
Sequence domains: Poly(ADP-ribose) polymerase catalytic domain
Structure domains: Phosphoenolpyruvate Carboxykinase; domain 3

Ligands and Environments

4 bound ligands:
Ligand ZN 4 x ZN
Ligand G18 3 x G18
Ligand GOL 4 x GOL
Ligand EOH 1 x EOH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 74.06Å b: 79.41Å c: 153.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.182 0.208
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Regulation of Wnt/β-catenin signalling by tankyrase-dependent poly(ADP-ribosyl)ation and scaffolding.
Mariotti et al. (2017)
4 more

1 mention without citation

Insights into the binding of PARP inhibitors to the catalytic domain of human tankyrase-2.
Qiu et al. (2014)