PDBe 4pky

X-ray diffraction
3.2Å resolution

ARNT/HIF transcription factor/coactivator complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
monomeric
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Aryl hydrocarbon receptor nuclear translocator Chains: A, D
Molecule details ›
Chains: A, D
Length: 121 amino acids
Theoretical weight: 14.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27540 (Residues: 356-470; Coverage: 15%)
Gene names: ARNT, BHLHE2
Sequence domains: PAS domain
Structure domains: Beta-Lactamase
Transforming acidic coiled-coil-containing protein 3 Chains: B, C, E, F
Molecule details ›
Chains: B, C, E, F
Length: 50 amino acids
Theoretical weight: 5.81 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9JJ11 (Residues: 585-631; Coverage: 7%)
Gene names: Aint, Tacc3
Endothelial PAS domain-containing protein 1 Chain: G
Molecule details ›
Chain: G
Length: 117 amino acids
Theoretical weight: 13.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99814 (Residues: 239-350; Coverage: 13%)
Gene names: BHLHE73, EPAS1, HIF2A, MOP2, PASD2
Sequence domains: PAS fold
Structure domains: Beta-Lactamase

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 96.136Å b: 96.136Å c: 182.841Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.231 0.27
Expression system: Escherichia coli BL21(DE3)