› 4pg5

X-ray diffraction
2.2Å resolution

Crystal structure of S. aureus Homoserine Dehydrogenase at pH6.5

Released:
DOI: 10.2210/pdb4pg5/pdb
PDB entry 4pg5 coloured by chain, front view.
PDB entry 4pg5 coloured by chain, side view.
PDB entry 4pg5 coloured by chain, top view.
Source organism: Staphylococcus aureus M1064
Primary publication:
Structural basis for the catalytic mechanism of homoserine dehydrogenase.
Navratna V, Reddy G, Gopal B
Acta Crystallogr D Biol Crystallogr 71 1216-25 (2015)
PMID: 25945586

Function and Biology Details

Reaction catalysed: 
L-homoserine + NAD(P)(+) = L-aspartate 4-semialdehyde + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homoserine dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 468 amino acids
Theoretical weight: 51.38 KDa
Source organism: Staphylococcus aureus M1064
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0M3KKV6 (Residues: 1-426; Coverage: 95%)
Gene name: U5K_01898
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ACT 11 x ACT
Ligand GOL 2 x GOL
Ligand PEG 2 x PEG
Ligand DMS 4 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 72.48Å b: 115.43Å c: 118.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.238
Expression system: Escherichia coli

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Citations

5 citation in other articles

l-lysine production by Bacillus methanolicus: Genome-based mutational analysis and l-lysine secretion engineering.
Nærdal et al. (2017)
4 more