› 4pes

X-ray diffraction
2.21Å resolution

Crystal structure of insulin degrading enzyme complexed with inhibitor tert-butyl [(2S)-2-(2,5-difluorophenyl)-3-(quinolin-3-yl)propyl]carbamate

Released:
DOI: 10.2210/pdb4pes/pdb
PDB entry 4pes coloured by chain, front view.
PDB entry 4pes coloured by chain, side view.
PDB entry 4pes coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Wang Y, Guo S

Function and Biology Details

Reaction catalysed: 
Degradation of insulin, glucagon and other polypeptides. No action on proteins.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147042 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin-degrading enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 989 amino acids
Theoretical weight: 114.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14735 (Residues: 42-1019; Coverage: 96%)
Gene name: IDE
Sequence domains:
Structure domains: Metalloenzyme, LuxS/M16 peptidase-like
Ala-Ala-Ala Chains: C, D
Molecule details ›
Chains: C, D
Length: 3 amino acids
Theoretical weight: 231 Da
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand 2PJ 2 x 2PJ
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 78.754Å b: 116.087Å c: 123.923Å
α: 90° β: 98.09° γ: 90°
R-values:
R R work R free
0.209 0.209 0.256
Expression system: Escherichia coli

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