› 4otp

X-ray diffraction
2.7Å resolution

Crystal structure of the catalytic domain of the human RioK1 atypical protein kinase in complex with ADP/Mg2+

Released:
DOI: 10.2210/pdb4otp/pdb
PDB entry 4otp coloured by chain, front view.
PDB entry 4otp coloured by chain, side view.
PDB entry 4otp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Dominant Rio1 kinase/ATPase catalytic mutant induces trapping of late pre-40S biogenesis factors in 80S-like ribosomes.
Ferreira-Cerca S, Kiburu I, Thomson E, LaRonde N, Hurt E
Nucleic Acids Res 42 8635-47 (2014)
PMID: 24948609

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189693 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase RIO1 Chain: A
Molecule details ›
Chain: A
Length: 352 amino acids
Theoretical weight: 41.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BRS2 (Residues: 143-494; Coverage: 62%)
Gene names: RIO1, RIOK1
Sequence domains: RIO1 family
Structure domains: Transferase(Phosphotransferase) domain 1

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 1 x MG
2 modified residues:
Ligand MSE 17 x MSE
Ligand PHD 1 x PHD

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3221
Unit cell:
a: 78.76Å b: 78.76Å c: 110.57Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.204 0.243
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Functions of ribosomal proteins in assembly of eukaryotic ribosomes in vivo.
de la Cruz et al. (2015)
11 more

9 mentions without citation

The Link between Protein Kinase CK2 and Atypical Kinase Rio1.
Kubiński et al. (2017)
8 more