› 4njg

X-ray diffraction
2.6Å resolution

Crystal Structure of QueE from Burkholderia multivorans in complex with AdoMet and 6-carboxypterin

Released:
DOI: 10.2210/pdb4njg/pdb
PDB entry 4njg coloured by chain, front view.
PDB entry 4njg coloured by chain, side view.
PDB entry 4njg coloured by chain, top view.
Source organism: Burkholderia multivorans ATCC 17616
Primary publication:
Radical SAM enzyme QueE defines a new minimal core fold and metal-dependent mechanism.
Dowling DP, Bruender NA, Young AP, McCarty RM, Bandarian V, Drennan CL
Nat Chem Biol 10 106-12 (2014)
PMID: 24362703

Function and Biology Details

Reaction catalysed: 
6-carboxy-5,6,7,8-tetrahydropterin = 7-carboxy-7-carbaguanine + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102048 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
7-carboxy-7-deazaguanine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 25.34 KDa
Source organism: Burkholderia multivorans ATCC 17616
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3KB22 (Residues: 1-210; Coverage: 100%)
Gene names: BMULJ_00116, Bmul_3115, queE
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand SAM 2 x SAM
2 bound ligands:
Ligand SF4 2 x SF4
Ligand HHS 2 x HHS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P43212
Unit cell:
a: 118.956Å b: 118.956Å c: 101.952Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.183 0.21
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Radical S-adenosylmethionine enzymes.
Broderick et al. (2014)
11 more

4 mentions without citation

Radical-mediated ring contraction in the biosynthesis of 7-deazapurines.
Bandarian et al. (2015)
3 more