› 4nik

X-ray diffraction
2.5Å resolution

Structure of human Gankyrin in complex to the single chain antibody F5

Released:
DOI: 10.2210/pdb4nik/pdb
PDB entry 4nik coloured by chain, front view.
PDB entry 4nik coloured by chain, side view.
PDB entry 4nik coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Restricted diversity of antigen binding residues of antibodies revealed by computational alanine scanning of 227 antibody-antigen complexes.
Robin G, Sato Y, Desplancq D, Rochel N, Weiss E, Martineau P
J Mol Biol 426 3729-3743 (2014)
PMID: 25174334

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-234997 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
26S proteasome non-ATPase regulatory subunit 10 Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 24.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75832 (Residues: 1-226; Coverage: 100%)
Gene name: PSMD10
Sequence domains: Ankyrin repeats (3 copies)
Structure domains: Ankyrin repeat-containing domain
Single-chain Fv fragment antibody Chain: B
Molecule details ›
Chain: B
Length: 256 amino acids
Theoretical weight: 26.86 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P3221
Unit cell:
a: 135.578Å b: 135.578Å c: 68.655Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.166 0.164 0.208
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Breaking the law: unconventional strategies for antibody diversification.
Kanyavuz et al. (2019)
5 more

4 mentions without citation

The Effect of Mutations in the TPR and Ankyrin Families of Alpha Solenoid Repeat Proteins.
Izert et al. (2021)
3 more