PDBe 4nd4

X-ray diffraction
2.2Å resolution

Crystal structure of the lactate dehydrogenase from cryptosporidium parvum complexed with substrate (pyruvic acid) and cofactor (b-nicotinamide adenine dinucleotide)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactate dehydrogenase, adjacent gene encodes predicted malate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 321 amino acids
Theoretical weight: 33.88 KDa
Source organism: Cryptosporidium parvum Iowa II
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5CYZ2 (Residues: 17-337; Coverage: 95%)
Gene name: cgd7_480
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P3221
Unit cell:
a: 95.905Å b: 95.905Å c: 185.723Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.201 0.223
Expression system: Escherichia coli