› 4nab

X-ray diffraction
3.5Å resolution

Structure of the (SR)Ca2+-ATPase mutant E309Q in the Ca2-E1-MgAMPPCP form

Released:
DOI: 10.2210/pdb4nab/pdb
PDB entry 4nab coloured by chain, front view.
PDB entry 4nab coloured by chain, side view.
PDB entry 4nab coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
SERCA mutant E309Q binds two Ca(2+) ions but adopts a catalytically incompetent conformation.
Clausen JD, Bublitz M, Arnou B, Montigny C, Jaxel C, Møller JV, Nissen P, Andersen JP, le Maire M
EMBO J 32 3231-43 (2013)
PMID: 24270570

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + Ca(2+)(Side 1) = ADP + phosphate + Ca(2+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137501 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 Chain: A
Molecule details ›
Chain: A
Length: 1000 amino acids
Theoretical weight: 110.18 KDa
Source organism: Oryctolagus cuniculus
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P04191 (Residues: 1-993; Coverage: 99%)
Gene name: ATP2A1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand PCW 1 x PCW
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 167.01Å b: 55.79Å c: 161.79Å
α: 90° β: 109.29° γ: 90°
R-values:
R R work R free
0.218 0.216 0.266
Expression system: Saccharomyces cerevisiae

Quick links

Citations

1 review citation

General and specific interactions of the phospholipid bilayer with P-type ATPases.
Hossain et al. (2019)
24 other articles

5 mentions without citation

Linking Biochemical and Structural States of SERCA: Achievements, Challenges, and New Opportunities.
Aguayo-Ortiz et al. (2020)
4 more