PDBe 4mny

X-ray diffraction
1.7Å resolution

Crystal structure of urokinase-type plasminogen activator (uPA) complexed with bicyclic peptide UK903

Primary publication:
Peptide Ligands Stabilized by Small Molecules.
Angew. Chem. Int. Ed. Engl. (2014)
PMID: 24453110

Function and Biology Details

Reaction catalysed:
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
bicyclic peptide UK903 Chains: C, D
Molecule details ›
Chains: C, D
Length: 14 amino acids
Theoretical weight: 1.39 KDa
Source organism: Synthetic construct
Expression system: Not provided
Urokinase-type plasminogen activator chain B Chains: A, B
Molecule details ›
Chains: A, B
Length: 245 amino acids
Theoretical weight: 27.59 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
  • Canonical: P00749 (Residues: 179-423; Coverage: 60%)
Gene name: PLAU
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 67.959Å b: 54.457Å c: 71.681Å
α: 90° β: 98.18° γ: 90°
R R work R free
0.168 0.166 0.215
Expression systems:
  • Not provided
  • Homo sapiens