› 4mnl

X-ray diffraction
1.6Å resolution

Crystal Structure of GH18 Chitinase (G77W/E119Q mutant) from Cycas revoluta in complex with (GlcNAc)4

Released:
DOI: 10.2210/pdb4mnl/pdb
PDB entry 4mnl coloured by chain, front view.
PDB entry 4mnl coloured by chain, side view.
PDB entry 4mnl coloured by chain, top view.
Source organism: Cycas revoluta
Entry authors: Umemoto N, Numata T, Ohnuma T, Osawa T, Taira T, Fukamizo T

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175790 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
GH18 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 38.73 KDa
Source organism: Cycas revoluta
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4W6L6 (Residues: 24-370; Coverage: 95%)
Gene name: crchiA
Sequence domains: Glycosyl hydrolases family 18
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 57.478Å b: 64.109Å c: 85.838Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.199 0.218
Expression system: Escherichia coli

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