› 4mk0

X-ray diffraction
2.4Å resolution

Crystal structure of G protein-coupled receptor kinase 2 in complex with a a rationally designed paroxetine derivative

Released:
DOI: 10.2210/pdb4mk0/pdb
PDB entry 4mk0 coloured by chain, front view.
PDB entry 4mk0 coloured by chain, side view.
PDB entry 4mk0 coloured by chain, top view.
Source organisms:
Primary publication:
Structural and functional analysis of g protein-coupled receptor kinase inhibition by paroxetine and a rationally designed analog.
Homan KT, Wu E, Wilson MW, Singh P, Larsen SD, Tesmer JJ
Mol Pharmacol 85 237-48 (2014)
PMID: 24220010

Function and Biology Details

Reaction catalysed: 
ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-150375 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Beta-adrenergic receptor kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 640 amino acids
Theoretical weight: 74.55 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P25098 (Residues: 30-668; Coverage: 93%)
Gene names: ADRBK1, BARK, BARK1, GRK2
Sequence domains:
Structure domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 339 amino acids
Theoretical weight: 37.29 KDa
Source organism: Bos taurus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62871 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 59 amino acids
Theoretical weight: 6.52 KDa
Source organism: Bos taurus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63212 (Residues: 6-64; Coverage: 83%)
Gene name: GNG2
Sequence domains: GGL domain
Structure domains: Transducin (heterotrimeric G protein), gamma chain

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand 29X 1 x 29X
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2221
Unit cell:
a: 61.222Å b: 240.973Å c: 212.083Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.175 0.238
Expression system: Trichoplusia ni

Quick links

Citations

9 review citations

Structural insights into G protein-coupled receptor kinase function.
Homan et al. (2014)
8 more

5 mentions without citation

The RAF Kinase Inhibitor Protein (RKIP): Good as Tumour Suppressor, Bad for the Heart.
Abd Alla et al. (2022)
4 more