PDBe 4mfa

X-ray diffraction
2.27Å resolution

Structure of human DNA polymerase beta complexed with nicked DNA containing a mismatched template O6MG and incoming TTP

Released:
Source organism: Homo sapiens
Entry authors: Koag MC, Min K, Monzingo AF, Lee S

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase beta Chain: A
Molecule details ›
Chain: A
Length: 325 amino acids
Theoretical weight: 37.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06746 (Residues: 11-335; Coverage: 97%)
Gene name: POLB
Sequence domains:
Structure domains:
template Chain: T
Molecule details ›
Chain: T
Length: 16 nucleotides
Theoretical weight: 4.86 KDa
Source organism: Homo sapiens
Expression system: Not provided
up primer Chain: P
Molecule details ›
Chain: P
Length: 11 nucleotides
Theoretical weight: 3.39 KDa
Source organism: Homo sapiens
Expression system: Not provided
dn primer Chain: D
Molecule details ›
Chain: D
Length: 5 nucleotides
Theoretical weight: 1.54 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 54.971Å b: 79.662Å c: 55.001Å
α: 90° β: 106.68° γ: 90°
R-values:
R R work R free
0.216 0.212 0.283
Expression systems:
  • Escherichia coli
  • Not provided