› 4mdp

X-ray diffraction
2.05Å resolution

Crystal structure of a GH1 beta-glucosidase from the fungus Humicola insolens in complex with glucose

Released:
DOI: 10.2210/pdb4mdp/pdb
PDB entry 4mdp coloured by chain, front view.
PDB entry 4mdp coloured by chain, side view.
PDB entry 4mdp coloured by chain, top view.
Source organism: [Humicola] grisea var. thermoidea
Primary publication:
Structural basis for glucose tolerance in GH1 β-glucosidases.
de Giuseppe PO, Souza Tde A, Souza FH, Zanphorlin LM, Machado CB, Ward RJ, Jorge JA, Furriel Rdos P, Murakami MT
Acta Crystallogr D Biol Crystallogr 70 1631-9 (2014)
PMID: 24914974

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131687 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucosidase Chain: A
Molecule details ›
Chain: A
Length: 499 amino acids
Theoretical weight: 56.59 KDa
Source organism: [Humicola] grisea var. thermoidea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O93784 (Residues: 1-476; Coverage: 100%)
Gene name: bgl4
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

4 bound ligands:
Ligand BGC 1 x BGC
Ligand PEG 3 x PEG
Ligand PO4 2 x PO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P6522
Unit cell:
a: 113.567Å b: 113.567Å c: 178.74Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.165 0.163 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Genetic modification: A tool for enhancing beta-glucosidase production for biofuel application.
Singhania et al. (2017)
38 other articles

6 mentions without citation

Oligomerization as a strategy for cold adaptation: Structure and dynamics of the GH1 β-glucosidase from Exiguobacterium antarcticum B7.
Zanphorlin et al. (2016)
5 more