PDBe 4m9n

X-ray diffraction
2.28Å resolution

DNA Polymerase Beta E295K Soaked with dATP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase beta Chain: A
Molecule details ›
Chain: A
Length: 335 amino acids
Theoretical weight: 38.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06746 (Residues: 1-335; Coverage: 100%)
Gene name: POLB
Sequence domains:
Structure domains:
DNA Primer Strand Chain: P
Molecule details ›
Chain: P
Length: 10 nucleotides
Theoretical weight: 3.06 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA Template Strand Chain: T
Molecule details ›
Chain: T
Length: 16 nucleotides
Theoretical weight: 4.85 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA Downstream Strand Chain: D
Molecule details ›
Chain: D
Length: 5 nucleotides
Theoretical weight: 1.54 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21
Unit cell:
a: 54.523Å b: 79.215Å c: 54.685Å
α: 90° β: 106.24° γ: 90°
R-values:
R R work R free
0.195 0.192 0.227
Expression systems:
  • Escherichia coli
  • Not provided