PDBe 4m75

X-ray diffraction
2.95Å resolution

Crystal structure of Lsm1-7 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Sm-like protein LSm1 Chains: A, H
Molecule details ›
Chains: A, H
Length: 144 amino acids
Theoretical weight: 17.47 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P47017 (Residues: 30-172; Coverage: 83%)
Gene names: J0714, LSM1, SPB8, YJL124C
Sequence domains: LSM domain
U6 snRNA-associated Sm-like protein LSm2 Chains: B, I
Molecule details ›
Chains: B, I
Length: 95 amino acids
Theoretical weight: 11.3 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P38203 (Residues: 1-95; Coverage: 100%)
Gene names: LSM2, SMX5, SNP3, YBL026W, YBL0425
Sequence domains: LSM domain
U6 snRNA-associated Sm-like protein LSm3 Chains: C, J
Molecule details ›
Chains: C, J
Length: 89 amino acids
Theoretical weight: 10.1 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P57743 (Residues: 1-89; Coverage: 100%)
Gene names: LSM3, SMX4, USS2, YLR438C-A
Sequence domains: LSM domain
Structure domains: SH3 type barrels.
U6 snRNA-associated Sm-like protein LSm4 Chains: G, N
Molecule details ›
Chains: G, N
Length: 122 amino acids
Theoretical weight: 14.09 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P40070 (Residues: 1-93; Coverage: 50%)
Gene names: LSM4, SDB23, USS1, YER112W
Sequence domains: LSM domain
Structure domains: SH3 type barrels.
U6 snRNA-associated Sm-like protein LSm5 Chains: E, L
Molecule details ›
Chains: E, L
Length: 93 amino acids
Theoretical weight: 10.57 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P40089 (Residues: 1-93; Coverage: 100%)
Gene names: LSM5, YER146W
Sequence domains: LSM domain
Structure domains: SH3 type barrels.
U6 snRNA-associated Sm-like protein LSm6 Chains: D, K
Molecule details ›
Chains: D, K
Length: 86 amino acids
Theoretical weight: 9.55 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06406 (Residues: 1-86; Coverage: 100%)
Gene names: D9481.18, LSM6, YDR378C
Sequence domains: LSM domain
Structure domains: SH3 type barrels.
U6 snRNA-associated Sm-like protein LSm7 Chains: F, M
Molecule details ›
Chains: F, M
Length: 115 amino acids
Theoretical weight: 13.26 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P53905 (Residues: 1-115; Coverage: 100%)
Gene names: LSM7, N1202, N1780, YNL147W
Sequence domains: LSM domain
Structure domains: SH3 type barrels.

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 66.672Å b: 67.835Å c: 98.235Å
α: 93.14° β: 108.21° γ: 116.3°
R-values:
R R work R free
0.267 0.266 0.291
Expression system: Escherichia coli