› 4m3h

X-ray diffraction
2.2Å resolution

Crystal structure of small laccase Ssl1 from Streptomyces sviceus

Released:
DOI: 10.2210/pdb4m3h/pdb
PDB entry 4m3h coloured by chain, front view.
PDB entry 4m3h coloured by chain, side view.
PDB entry 4m3h coloured by chain, top view.
Source organism: Streptomyces sviceus
Primary publication:
Structural and redox properties of the small laccase Ssl1 from Streptomyces sviceus.
Gunne M, Höppner A, Hagedoorn PL, Urlacher VB
FEBS J 281 4307-18 (2014)
PMID: 24548692

Function and Biology Details

Reaction catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110210 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper oxidase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 325 amino acids
Theoretical weight: 35.62 KDa
Source organism: Streptomyces sviceus
Expression system: Escherichia coli
UniProt:
  • Canonical: B5HSR1 (Residues: 8-325; Coverage: 98%)
Gene name: SSEG_02446
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU 11 x CU
Ligand O 2 x O
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 51.54Å b: 103.82Å c: 162.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.268
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Laccase Properties, Physiological Functions, and Evolution.
Janusz et al. (2020)
9 more

6 mentions without citation

Structural and redox properties of the small laccase Ssl1 from Streptomyces sviceus.
Gunne et al. (2014)
5 more