› 4m1n

X-ray diffraction
1.5Å resolution

Crystal structure of Plasmodium falciparum ubiquitin conjugating enzyme UBC9

Released:
DOI: 10.2210/pdb4m1n/pdb
PDB entry 4m1n coloured by chain, front view.
PDB entry 4m1n coloured by chain, side view.
PDB entry 4m1n coloured by chain, top view.
Source organism: Plasmodium falciparum 3D7
Primary publication:
Identification of biochemically distinct properties of the small ubiquitin-related modifier (SUMO) conjugation pathway in Plasmodium falciparum.
Reiter K, Mukhopadhyay D, Zhang H, Boucher LE, Kumar N, Bosch J, Matunis MJ
J Biol Chem 288 27724-36 (2013)
PMID: 23943616

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184651 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UBC core domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 160 amino acids
Theoretical weight: 18.29 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8I301 (Residues: 2-159; Coverage: 99%)
Gene name: PF3D7_0915100
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2221
Unit cell:
a: 85.19Å b: 90.77Å c: 122.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.196
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Post-translational modifications as key regulators of apicomplexan biology: insights from proteome-wide studies.
Yakubu et al. (2018)
3 more

4 mentions without citation

Targeting SUMOylation in Plasmodium as a Potential Target for Malaria Therapy.
Sumam de Oliveira et al. (2021)
3 more