› 4lks

X-ray diffraction
1.5Å resolution

Structure of CBM32-3 from a family 31 glycoside hydrolase from Clostridium perfringens in complex with galactose

Released:
DOI: 10.2210/pdb4lks/pdb
PDB entry 4lks coloured by chain, front view.
PDB entry 4lks coloured by chain, side view.
PDB entry 4lks coloured by chain, top view.
Source organism: Clostridium perfringens ATCC 13124
Primary publication:
Diverse modes of galacto-specific carbohydrate recognition by a family 31 glycoside hydrolase from Clostridium perfringens.
Grondin JM, Duan D, Kirlin AC, Abe KT, Chitayat S, Spencer HL, Spencer C, Campigotto A, Houliston S, Arrowsmith CH, Allingham JS, Boraston AB, Smith SP
PLoS One 12 e0171606 (2017)
PMID: 28158290

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101678 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase, family 31/fibronectin type III domain protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 167 amino acids
Theoretical weight: 18.99 KDa
Source organism: Clostridium perfringens ATCC 13124
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2YST8 (Residues: 1640-1785; Coverage: 7%)
Gene name: CPF_1301
Sequence domains: F5/8 type C domain
Structure domains: Galactose-binding domain-like

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand GAL 2 x GAL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P212121
Unit cell:
a: 62.338Å b: 62.325Å c: 85.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.175 0.201
Expression system: Escherichia coli

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Citations

6 citation in other articles

Systematic Review of Gut Microbiota and Major Depression.
Cheung et al. (2019)
5 more

2 mentions without citation

Complexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition.
Venditto et al. (2016)
1 more