PDBe 4lgp

X-ray diffraction
2.4Å resolution

Function and Biology Details

Reaction catalysed:
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ricin A chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 261 amino acids
Theoretical weight: 29.29 KDa
Source organism: Ricinus communis
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P02879 (Residues: 36-296; Coverage: 48%)
Sequence domains: Ribosome inactivating protein
Structure domains:
VHH1 camelid nanobody Chains: B, D
Molecule details ›
Chains: B, D
Length: 116 amino acids
Theoretical weight: 12.54 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 90.168Å b: 92.443Å c: 93.317Å
α: 90° β: 90° γ: 90°
R R work R free
0.228 0.225 0.28
Expression system: Escherichia coli BL21(DE3)