› 4lg6

X-ray diffraction
1.8Å resolution

Crystal structure of ANKRA2-CCDC8 complex

Released:
DOI: 10.2210/pdb4lg6/pdb
PDB entry 4lg6 coloured by chain, front view.
PDB entry 4lg6 coloured by chain, side view.
PDB entry 4lg6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Ankyrin repeats of ANKRA2 recognize a PxLPxL motif on the 3M syndrome protein CCDC8.
Nie J, Xu C, Jin J, Aka JA, Tempel W, Nguyen V, You L, Weist R, Min J, Pawson T, Yang XJ
Structure 23 700-12 (2015)
PMID: 25752541

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190498 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ankyrin repeat family A protein 2 Chain: A
Molecule details ›
Chain: A
Length: 173 amino acids
Theoretical weight: 18.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9H9E1 (Residues: 142-313; Coverage: 55%)
Gene names: ANKRA, ANKRA2
Sequence domains:
Structure domains: Ankyrin repeat-containing domain
Coiled-coil domain-containing protein 8 Chain: B
Molecule details ›
Chain: B
Length: 17 amino acids
Theoretical weight: 2.08 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9H0W5 (Residues: 494-510; Coverage: 3%)
Gene name: CCDC8

Ligands and Environments

1 bound ligand:
Ligand UNX 3 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E
Spacegroup: P21
Unit cell:
a: 38.726Å b: 49.791Å c: 41.243Å
α: 90° β: 98.23° γ: 90°
R-values:
R R work R free
0.197 0.196 0.208
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

2 review citations

Cullin-RING E3 Ubiquitin Ligase 7 in Growth Control and Cancer.
Pan ZQ. (2020)
1 more