› 4lfi

X-ray diffraction
1.85Å resolution

Crystal structure of scCK2 alpha in complex with GMPPNP

Released:
DOI: 10.2210/pdb4lfi/pdb
PDB entry 4lfi coloured by chain, front view.
PDB entry 4lfi coloured by chain, side view.
PDB entry 4lfi coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
The multiple nucleotide-divalent cation binding modes of Saccharomyces cerevisiae CK2α indicate a possible co-substrate hydrolysis product (ADP/GDP) release pathway.
Liu H, Wang H, Teng M, Li X
Acta Crystallogr D Biol Crystallogr 70 501-13 (2014)
PMID: 24531484

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147483 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Casein kinase II subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 44.93 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P15790 (Residues: 1-372; Coverage: 100%)
Gene names: CKA1, YIL035C
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand GNP 2 x GNP
Ligand MN 4 x MN
Ligand GOL 2 x GOL
Ligand SO4 10 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P32
Unit cell:
a: 69.664Å b: 69.664Å c: 166.251Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.171 0.2
Expression system: Escherichia coli

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Citations

1 review citation

Emerging roles of the αC-β4 loop in protein kinase structure, function, evolution, and disease.
Yeung et al. (2020)
2 other articles