› 4l36

X-ray diffraction
2.1Å resolution

Crystal structure of the cytochrome P450 enzyme TxtE

Released:
DOI: 10.2210/pdb4l36/pdb
PDB entry 4l36 coloured by chain, front view.
PDB entry 4l36 coloured by chain, side view.
PDB entry 4l36 coloured by chain, top view.
Source organism: Streptomyces scabiei 87.22
Primary publication:
Structural insights into the mechanism for recognizing substrate of the cytochrome P450 enzyme TxtE.
Yu F, Li M, Xu C, Wang Z, Zhou H, Yang M, Chen Y, Tang L, He J
PLoS One 8 e81526 (2013)
PMID: 24282603

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111896 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative P450-like protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 426 amino acids
Theoretical weight: 47 KDa
Source organism: Streptomyces scabiei 87.22
Expression system: Escherichia coli
UniProt:
  • Canonical: C9ZDC6 (Residues: 1-406; Coverage: 100%)
Gene name: SCAB_31831
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEB 2 x HEB
Ligand IMD 3 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 46.316Å b: 52.595Å c: 88.245Å
α: 77.76° β: 82.17° γ: 68.36°
R-values:
R R work R free
0.198 0.196 0.234
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Biosynthetic manipulation of tryptophan in bacteria: pathways and mechanisms.
Alkhalaf et al. (2015)
2 more

4 mentions without citation

Cytochromes P450 for natural product biosynthesis in Streptomyces: sequence, structure, and function.
Rudolf et al. (2017)
3 more