› 4kva

X-ray diffraction
2.14Å resolution

GTPase domain of Septin 10 from Schistosoma mansoni in complex with GTP

Released:
DOI: 10.2210/pdb4kva/pdb
PDB entry 4kva coloured by chain, front view.
PDB entry 4kva coloured by chain, side view.
PDB entry 4kva coloured by chain, top view.
Source organism: Schistosoma mansoni
Primary publication:
Crystal structure of a Schistosoma mansoni septin reveals the phenomenon of strand slippage in septins dependent on the nature of the bound nucleotide.
Zeraik AE, Pereira HM, Santos YV, Brandão-Neto J, Spoerner M, Santos MS, Colnago LA, Garratt RC, Araújo AP, DeMarco R
J Biol Chem 289 7799-811 (2014)
PMID: 24464615

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104926 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Septin-10 Chains: A, B
Molecule details ›
Chains: A, B
Length: 412 amino acids
Theoretical weight: 48.16 KDa
Source organism: Schistosoma mansoni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A3Q0KDV9 (Residues: 1-412; Coverage: 100%)
Gene names: SEPT10, Smp_029890
Sequence domains: Septin
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2
Unit cell:
a: 160.8Å b: 47.04Å c: 95.48Å
α: 90° β: 112.61° γ: 90°
R-values:
R R work R free
0.187 0.187 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The Mammalian Septin Interactome.
Neubauer et al. (2017)
1 more

5 mentions without citation

Septin structure and filament assembly.
Valadares et al. (2017)
4 more