› 4kt7

X-ray diffraction
2Å resolution

The crystal structure of 4-diphosphocytidyl-2C-methyl-D-erythritolsynthase from Anaerococcus prevotii DSM 20548

Released:
DOI: 10.2210/pdb4kt7/pdb
PDB entry 4kt7 coloured by chain, front view.
PDB entry 4kt7 coloured by chain, side view.
PDB entry 4kt7 coloured by chain, top view.
Source organism: Anaerococcus prevotii DSM 20548
Entry authors: Borek D, Tan K, Stols L, Eschenfeidt WH, Otwinoski Z, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-111759 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 237 amino acids
Theoretical weight: 27.45 KDa
Source organism: Anaerococcus prevotii DSM 20548
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C7RFG6 (Residues: 1-234; Coverage: 100%)
Gene name: Apre_0175
Sequence domains: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand NA 1 x NA
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 87.858Å b: 145.509Å c: 36.719Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.228
Expression system: Escherichia coli BL21(DE3)

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