PDBe 4km4

X-ray diffraction
2.8Å resolution

E. coli alkaline phosphatase mutant S102G/R166S in complex with inorganic phosphate

Released:

Function and Biology Details

Reaction catalysed:
A phosphate monoester + H(2)O = an alcohol + phosphate. 
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkaline phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 445 amino acids
Theoretical weight: 46.54 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P00634 (Residues: 26-470; Coverage: 99%)
Gene names: JW0374, b0383, phoA
Sequence domains: Alkaline phosphatase
Structure domains: Alkaline Phosphatase, subunit A

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P6322
Unit cell:
a: 160.935Å b: 160.935Å c: 139.549Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.232 0.296
Expression system: Escherichia coli