PDBe 4kji

X-ray diffraction
3.2Å resolution

Novel re-arrangement of an RsmA/cSRa family protein to create a structurally distinct new RNA-binding family member

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RsmN, a RNA-binding protein of Regulator of Secondary Metabolism Chains: A, B
Molecule details ›
Chains: A, B
Length: 79 amino acids
Theoretical weight: 8.77 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2ZIZ8 (Residues: 1-71; Coverage: 100%)
Gene name: PA14_68470
Sequence domains: Global regulator protein family
RsmZ-2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 16 nucleotides
Theoretical weight: 5.19 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P4322
Unit cell:
a: 83.649Å b: 83.649Å c: 94.239Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.222 0.317
Expression systems:
  • Escherichia coli
  • Not provided