4kcf Citations

Active Site Architecture of a Sugar N-Oxygenase.

Biochemistry (2013)
Cited: 6 times
EuropePMC logo PMID: 23621882


KijD3 is a flavin-dependent N-oxygenase implicated in the formation of the nitro-containing sugar d-kijanose, found attached to the antibiotic kijanimicin. For this investigation, the structure of KijD3 in complex with FMN and its dTDP-sugar substrate was solved to 2.1 Å resolution. In contrast to the apoenzyme structure, the C-terminus of the protein becomes ordered and projects into the active site cleft [Bruender, N. A., Thoden, J. B., and Holden, H. M. (2010) Biochemistry 49, 3517-3524]. The amino group of the dTDP-aminosugar that is oxidized is located 4.9 Å from C4a of the flavin ring. The model provides a molecular basis for understanding the manner in which KijD3 catalyzes its unusual chemical transformation.

Articles citing this publication (6)

  1. Binding pockets and pathways for dioxygen through the KijD3 N-oxygenase in complex with flavin mononucleotide cofactor and a 3-aminoglucose substrate: predictions from molecular dynamics simulations. Pietra F. Chem. Biodivers. 11 1151-1162 (2014)
  2. Structural insights into the stabilization of active, tetrameric DszC by its C-terminus. Zhang L, Duan X, Zhou D, Dong Z, Ji K, Meng W, Li G, Li X, Yang H, Ma T, Rao Z. Proteins 82 2733-2743 (2014)
  3. Sphinganine-like biogenesis of (E)-1-nitropentadec-1-ene in termite soldiers of the genus Prorhinotermes. Jirošová A, Majer P, Jančařík A, Dolejšová K, Tykva R, Sobotník J, Jiroš P, Hanus R. Chembiochem 15 533-536 (2014)
  4. Structure of DnmZ, a nitrososynthase in the Streptomyces peucetius anthracycline biosynthetic pathway. Sartor L, Ibarra C, Al-Mestarihi A, Bachmann BO, Vey JL. Acta Crystallogr F Struct Biol Commun 71 1205-1214 (2015)
  5. Structural studies on KijD1, a sugar C-3'-methyltransferase. Dow GT, Thoden JB, Holden HM. Protein Sci. 25 2282-2289 (2016)
  6. Cryptic indole hydroxylation by a non-canonical terpenoid cyclase parallels bacterial xenobiotic detoxification. Kugel S, Baunach M, Baer P, Ishida-Ito M, Sundaram S, Xu Z, Groll M, Hertweck C. Nat Commun 8 15804 (2017)