PDBe 4kal

X-ray diffraction
1.5Å resolution

Crystal structure of probable sugar kinase protein from Rhizobium etli CFN 42 complexed with quinoline-3-carboxylic acid

Released:
Source organism: Rhizobium etli CFN 42
Entry authors: Malashkevich VN, Bhosle R, Toro R, Hillerich B, Gizzi A, Garforth S, Kar A, Chan MK, Lafluer J, Patel H, Matikainen B, Chamala S, Lim S, Celikgil A, Villegas G, Evans B, Love J, Fiser A, Khafizov K, Seidel R, Bonanno JB, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable sugar kinase protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 352 amino acids
Theoretical weight: 38.85 KDa
Source organism: Rhizobium etli CFN 42
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2KDX6 (Residues: 1-330; Coverage: 100%)
Gene name: RHE_CH00135
Sequence domains: pfkB family carbohydrate kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 51.181Å b: 81.219Å c: 82.95Å
α: 90° β: 98.17° γ: 90°
R-values:
R R work R free
0.16 0.159 0.19
Expression system: Escherichia coli BL21(DE3)