PDBe 4k98

X-ray diffraction
1.94Å resolution

Structure of Ternary Complex of cGAS with dsDNA and Bound 5 -pppG(2 ,5 )pG

Released:

Function and Biology Details

Reaction catalysed:
ATP + GTP = 2 diphosphate + cyclic G-P(2'-5')A-P(3'-5'). 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Cyclic GMP-AMP synthase Chain: A
Molecule details ›
Chain: A
Length: 362 amino acids
Theoretical weight: 42.51 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8C6L5 (Residues: 147-507; Coverage: 71%)
Gene name: Mb21d1
Sequence domains: Mab-21 protein
DNA-F Chain: D
Molecule details ›
Chain: D
Length: 17 nucleotides
Theoretical weight: 5.25 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA-R Chain: E
Molecule details ›
Chain: E
Length: 17 nucleotides
Theoretical weight: 5.16 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: I222
Unit cell:
a: 85.375Å b: 97.877Å c: 133.465Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.199
Expression systems:
  • Escherichia coli
  • Not provided