PDBe 4jnl

X-ray diffraction
2Å resolution

Crystal Structure of uPA in complex with its inhibitor 4-bromobenzylamine at pH 7.4

Released:
Source organism: Homo sapiens
Entry authors: Zhang X, Jiang L, Zhao B, Yuan C, Huang M

Function and Biology Details

Reaction catalysed:
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Urokinase-type plasminogen activator chain B Chain: U
Molecule details ›
Chain: U
Length: 247 amino acids
Theoretical weight: 27.84 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P00749 (Residues: 179-425; Coverage: 60%)
Gene name: PLAU
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: R3
Unit cell:
a: 121.575Å b: 121.575Å c: 42.212Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.198 0.252
Expression system: Saccharomyces cerevisiae