PDBe 4jgh

X-ray diffraction
3Å resolution

Structure of the SOCS2-Elongin BC complex bound to an N-terminal fragment of Cullin5

Released:
Source organisms:
Primary publication:
Structural basis of intersubunit recognition in elongin BC-cullin 5-SOCS box ubiquitin-protein ligase complexes.
Acta Crystallogr. D Biol. Crystallogr. 69 1587-97 (2013)
PMID: 23897481

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Suppressor of cytokine signaling 2 Chain: A
Molecule details ›
Chain: A
Length: 173 amino acids
Theoretical weight: 19.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14508 (Residues: 32-198; Coverage: 84%)
Gene names: CIS2, SOCS2, SSI2, STATI2
Sequence domains:
Structure domains:
Elongin-B Chain: B
Molecule details ›
Chain: B
Length: 118 amino acids
Theoretical weight: 13.19 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P62869 (Residues: 1-118; Coverage: 100%)
Gene names: Elob, Tceb2
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Elongin-C Chain: C
Molecule details ›
Chain: C
Length: 96 amino acids
Theoretical weight: 10.87 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P83940 (Residues: 17-112; Coverage: 86%)
Gene names: Eloc, Tceb1
Sequence domains: Skp1 family, tetramerisation domain
Structure domains: Potassium Channel Kv1.1; Chain A
Cullin-5 Chain: D
Molecule details ›
Chain: D
Length: 378 amino acids
Theoretical weight: 44.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93034 (Residues: 10-386; Coverage: 48%)
Gene names: CUL5, VACM1
Structure domains: Cullin Repeats

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: C2221
Unit cell:
a: 138.849Å b: 141.456Å c: 182.001Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.248
Expression system: Escherichia coli