4jd8 Citations

X-ray Crystal Structure of rac-[Ru(phen)2dppz](2+) with d(ATGCAT)2 Shows Enantiomer Orientations and Water Ordering.

J. Am. Chem. Soc. 135 12652-9 (2013)
Cited: 21 times
EuropePMC logo PMID: 23875832


We report an atomic resolution X-ray crystal structure containing both enantiomers of rac-[Ru(phen)2dppz](2+) with the d(ATGCAT)2 DNA duplex (phen = phenanthroline; dppz = dipyridophenazine). The first example of any enantiomeric pair crystallized with a DNA duplex shows different orientations of the Λ and Δ binding sites, separated by a clearly defined structured water monolayer. Job plots show that the same species is present in solution. Each enantiomer is bound at a TG/CA step and shows intercalation from the minor groove. One water molecule is directly located on one phenazine N atom in the Δ-enantiomer only.

Reviews - 4jd8 mentioned but not cited (1)

  1. Transition Metal Intercalators as Anticancer Agents-Recent Advances. Deo KM, Pages BJ, Ang DL, Gordon CP, Aldrich-Wright JR. Int J Mol Sci 17 (2016)

Articles citing this publication (20)

  1. Metal complex interactions with DNA. Pages BJ, Ang DL, Wright EP, Aldrich-Wright JR. Dalton Trans 44 3505-3526 (2015)
  2. Thread insertion of a bis(dipyridophenazine) diruthenium complex into the DNA double helix by the extrusion of AT base pairs and cross-linking of DNA duplexes. Boer DR, Wu L, Lincoln P, Coll M. Angew. Chem. Int. Ed. Engl. 53 1949-1952 (2014)
  3. From intercalation to groove binding: switching the DNA-binding mode of isostructural transition-metal complexes. Ahmad H, Wragg A, Cullen W, Wombwell C, Meijer AJ, Thomas JA. Chemistry 20 3089-3096 (2014)
  4. Reversal of a Single Base-Pair Step Controls Guanine Photo-Oxidation by an Intercalating Ruthenium(II) Dipyridophenazine Complex. Keane PM, Poynton FE, Hall JP, Sazanovich IV, Towrie M, Gunnlaugsson T, Quinn SJ, Cardin CJ, Kelly JM. Angew. Chem. Int. Ed. Engl. 54 8364-8368 (2015)
  5. Strong DNA deformation required for extremely slow DNA threading intercalation by a binuclear ruthenium complex. Almaqwashi AA, Paramanathan T, Lincoln P, Rouzina I, Westerlund F, Williams MC. Nucleic Acids Res. 42 11634-11641 (2014)
  6. Supramolecular activation of a molecular photocatalyst. Pfeffer MG, Pehlken C, Staehle R, Sorsche D, Streb C, Rau S. Dalton Trans 43 13307-13315 (2014)
  7. Monitoring guanine photo-oxidation by enantiomerically resolved Ru(II) dipyridophenazine complexes using inosine-substituted oligonucleotides. Keane PM, Poynton FE, Hall JP, Clark IP, Sazanovich IV, Towrie M, Gunnlaugsson T, Quinn SJ, Cardin CJ, Kelly JM. Faraday Discuss. 185 455-469 (2015)
  8. Binding Behaviors for Different Types of DNA G-Quadruplexes: Enantiomers of [Ru(bpy)2(L)](2+) (L=dppz, dppz-idzo). Shi S, Xu JH, Gao X, Huang HL, Yao TM. Chemistry 21 11435-11445 (2015)
  9. Dissecting the Dynamic Pathways of Stereoselective DNA Threading Intercalation. Almaqwashi AA, Andersson J, Lincoln P, Rouzina I, Westerlund F, Williams MC. Biophys. J. 110 1255-1263 (2016)
  10. Ru(II)-polypyridyl surface functionalised gold nanoparticles as DNA targeting supramolecular structures and luminescent cellular imaging agents. Martínez-Calvo M, Orange KN, Elmes RB, la Cour Poulsen B, Williams DC, Gunnlaugsson T. Nanoscale 8 563-574 (2016)
  11. Binding of Ru(terpyridine)(pyridine)dipyridophenazine to DNA studied with polarized spectroscopy and calorimetry. Mårtensson AK, Lincoln P. Dalton Trans 44 3604-3613 (2015)
  12. Guanine Can Direct Binding Specificity of Ru-dipyridophenazine (dppz) Complexes to DNA through Steric Effects. Hall JP, Gurung SP, Henle J, Poidl P, Andersson J, Lincoln P, Winter G, Sorensen T, Cardin DJ, Brazier JA, Cardin CJ. Chemistry 23 4981-4985 (2017)
  13. Luminescent ruthenium polypyridyl complexes with extended 'dppz' like ligands as DNA targeting binders and cellular agents. Poulsen BC, Estalayo-Adrián S, Blasco S, Bright SA, Kelly JM, Williams DC, Gunnlaugsson T. Dalton Trans 45 18208-18220 (2016)
  14. Ruthenium(ii) complexes with dppz: from molecular photoswitch to biological applications. Li G, Sun L, Ji L, Chao H. Dalton Trans 45 13261-13276 (2016)
  15. Study of picosecond processes of an intercalated dipyridophenazine Cr(III) complex bound to defined sequence DNAs using transient absorption and time-resolved infrared methods. Devereux SJ, Keane PM, Vasudevan S, Sazanovich IV, Towrie M, Cao Q, Sun XZ, George MW, Cardin CJ, Kane-Maguire NA, Kelly JM, Quinn SJ. Dalton Trans 43 17606-17609 (2014)
  16. Photochemically active DNA-intercalating ruthenium and related complexes - insights by combining crystallography and transient spectroscopy. Cardin CJ, Kelly JM, Quinn SJ. Chem Sci 8 4705-4723 (2017)
  17. Effect of the Ancillary Ligands on the Spectral Properties and G-Quadruplexes DNA Binding Behavior: A Combined Experimental and Theoretical Study. Shi S, Gao X, Huang H, Zhao J, Yao T. Chemistry 21 13390-13400 (2015)
  18. Photoinduced interactions of two dirhodium complexes with d(GTCGAC)2 probed by 2D NOESY. Palmer AM, Knoll JD, Turro C. Dalton Trans 44 3640-3646 (2015)
  19. The Structure of Linkers Affects the DNA Binding Properties of Tethered Dinuclear Ruthenium(II) Metallo-Intercalators. Saeed HK, Saeed IQ, Buurma NJ, Thomas JA. Chemistry 23 5467-5477 (2017)
  20. Inosine Can Increase DNA's Susceptibility to Photo-oxidation by a RuII Complex due to Structural Change in the Minor Groove. Keane PM, Hall JP, Poynton FE, Poulsen BC, Gurung SP, Clark IP, Sazanovich IV, Towrie M, Gunnlaugsson T, Quinn SJ, Cardin CJ, Kelly JM. Chemistry 23 10344-10351 (2017)