Structure analysis

Crystal structure of E. coli maltose transporter MalFGK2 in complex with its regulatory protein EIIAglc

X-ray diffraction
3.913Å resolution
Source organism: Escherichia coli K-12
Assembly composition:
hetero hexamer (preferred)
Entry contents: 4 distinct polypeptide molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero hexamer
Accessible surface area: 74998.7 Å2
Buried surface area: 22127.85 Å2
Dissociation area: 3,062.88 Å2
Dissociation energy (ΔGdiss): -0.79 kcal/mol
Dissociation entropy (TΔSdiss): 26.01 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-136376
Complex Portal ID: CPX-1978
Assembly 2
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Multimeric state: hetero hexamer
Accessible surface area: 75258.43 Å2
Buried surface area: 22140.43 Å2
Dissociation area: 5,466.42 Å2
Dissociation energy (ΔGdiss): 16.03 kcal/mol
Dissociation entropy (TΔSdiss): 41.19 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-136376
Complex Portal ID: CPX-1978

Macromolecules

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Chains: G, I
Length: 296 amino acids
Theoretical weight: 32.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P68183 (Residues: 1-296; Coverage: 100%)
Gene names: JW3992, b4032, malG
Pfam: Binding-protein-dependent transport system inner membrane component
InterPro:
CATH: MetI-like

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Chains: M, N, O, P
Length: 172 amino acids
Theoretical weight: 18.55 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P69783 (Residues: 1-169; Coverage: 100%)
Gene names: JW2410, b2417, crr, gsr, iex, tgs, treD
Pfam: phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1
InterPro:
CATH: Glucose Permease (Domain IIA)

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